mouse anti bovine igg2 monoclonal antibody  (Bio-Rad)

Journal: Parasite Immunology

Article Title: Allotype‐Dependent Responses to the Vaccine Candidate Thrombospondin‐Like Protein of Dictyocaulus viviparus in Calves

doi: 10.1111/pim.70013

Figure Lengend Snippet: IgG2 responses measured with mab of the vaccinated calves (left column) and the control calves (right column) against TLP (upper panel), TLP protein moiety (middle panel) and TLP glycan moiety (TLP minus TLP protein, lower panel). Filled arrow heads indicate time of vaccination and open arrow heads indicate day of challenge infection. OD correlates with worm counts * p < 0.05, ** p < 0.005.

Article Snippet: Total IgG2 ELISA with the monoclonal antibody was performed with the Bovine IgG2 ELISA quantification kit (Bethyl laboratories.inc.), but instead of the polyclonal conjugate, mouse anti‐IgG2 specific monoclonal antibody (Bio‐Rad, mca 626) was used (1:2000), followed by a 1:2000 goat antimouse Ig/AP (DAKO cat nr. D0486).

Techniques: Control, Glycoproteomics, Infection

Journal: Parasite Immunology

Article Title: Allotype‐Dependent Responses to the Vaccine Candidate Thrombospondin‐Like Protein of Dictyocaulus viviparus in Calves

doi: 10.1111/pim.70013

Figure Lengend Snippet: Total IgG2 in serum of Day 0, 28 and 70 from vaccinated (top) and control (bottom) calves. Measured with mab directed against bovine IgG2.

Article Snippet: Total IgG2 ELISA with the monoclonal antibody was performed with the Bovine IgG2 ELISA quantification kit (Bethyl laboratories.inc.), but instead of the polyclonal conjugate, mouse anti‐IgG2 specific monoclonal antibody (Bio‐Rad, mca 626) was used (1:2000), followed by a 1:2000 goat antimouse Ig/AP (DAKO cat nr. D0486).

Techniques: Control

Journal: Parasites & Vectors

Article Title: Vaccination of cattle with synthetic peptides corresponding to predicted extracellular domains of Rhipicephalus (Boophilus) microplus aquaporin 2 reduced the number of ticks feeding to repletion

doi: 10.1186/s13071-022-05166-1

Figure Lengend Snippet: Antibody titers across the experimental time course. Total IgG response to vaccination for each aquaporin peptide (peptides conjugated to KLH). Shown as the ratio of the absorbance at 450 nm between the pre-bleed sample and the sample at each time point. Sample dilution 1:256. Refer to Fig. for critical experimental time points (last vaccination at week 9, ticks dropping weeks 13–14)

Article Snippet: Specifically, the secondary antibodies were mouse anti-bovine IgG1 or mouse anti-bovine IgG2 (Bio-Rad Laboratories, Hercules, CA, USA) and the HRP conjugate was goat anti-mouse IgG (H+L) (Life Technologies, Frederick, MD, USA).

Techniques:

Journal: Parasites & Vectors

Article Title: Vaccination of cattle with synthetic peptides corresponding to predicted extracellular domains of Rhipicephalus (Boophilus) microplus aquaporin 2 reduced the number of ticks feeding to repletion

doi: 10.1186/s13071-022-05166-1

Figure Lengend Snippet: Antibody isotyping: stacked bar graph showing relative amount (absorbance at 450 nm) of IgG1 and IgG2 for each peptide from each calf. The ratio of absorbance of IgG1/IgG2 is shown below the graphs for each calf and each peptide

Article Snippet: Specifically, the secondary antibodies were mouse anti-bovine IgG1 or mouse anti-bovine IgG2 (Bio-Rad Laboratories, Hercules, CA, USA) and the HRP conjugate was goat anti-mouse IgG (H+L) (Life Technologies, Frederick, MD, USA).

Techniques:

Journal: Parasites & Vectors

Article Title: Vaccination of cattle with synthetic peptides corresponding to predicted extracellular domains of Rhipicephalus (Boophilus) microplus aquaporin 2 reduced the number of ticks feeding to repletion

doi: 10.1186/s13071-022-05166-1

Figure Lengend Snippet: Relative amounts of antibody isotypes IgG1 and IgG2

Article Snippet: Specifically, the secondary antibodies were mouse anti-bovine IgG1 or mouse anti-bovine IgG2 (Bio-Rad Laboratories, Hercules, CA, USA) and the HRP conjugate was goat anti-mouse IgG (H+L) (Life Technologies, Frederick, MD, USA).

Techniques: